Abstract: The long-term goal of bioengineered tissues is expected to achieve precise cell type distribution, physiological cell density, perfusable vascular channels, and mature functionality. However, fabricating an engineered tissue with the microenvironmental features of organs with a physiological cell density remains a significant challenge in this field due to the disturbances caused by printing vascular channels within pre-printed tissues, the need to maintain printing precision at high cell densities, and the inability of the suspension bath to provide a suitable growth environment. Herein, we modified the rheological properties of the bioactive hydrogel by incorporating thixotropic Laponite nanoclay (LPN), and demonstrated that an optimized ratio of collagen methacrylate (ColMA) to LPN forms a self-healing suspension bath, enhanced by hydrogen bonding interactions and capable of in situ crosslinking. This printing strategy was generalized as the embedded 3D printing in cell-dense suspension (EPICS). The self-healing properties of the EPICS remain unaffected even when encapsulating a near-physiological cell density of 10^8 cells/mL, and it provides precise control of printing resolution from 1 mm to 100 µm. Utilizing EPICS could create a robust hepatic model exhibiting mature liver markers and reduced apoptosis gene expression compared to the model containing 10^6 cells/mL. Moreover, EPICS can efficiently fabricate spatially controlled perfusable channels, thereby mimicking the spatially varied microenvironments of hepatocellular carcinoma, highlighting its broad applications in therapeutics involving tissue and organ constructs.