Abstract: Organoids provide powerful models for human development and disease, yet their growth, differentiation, and function are strongly influenced by mechanical cues that remain difficult to measure non-invasively. Understanding organoids mechanobiology requires methods that can quantify elasticity with high spatial resolution, longitudinal stability, and minimal perturbation. Here, we introduce an optical coherence elastography (OCE) platform based on spectral-domain optical coherence tomography that integrates controlled piezoelectric excitation with nanoscale, phase-resolved detection of elastic wave propagation. This system enables quantitative, non-destructive estimation of elastic modulus in soft tissues. Validation experiments using agar phantoms showed excellent agreement betwe

Abstract: The long-term goal of bioengineered tissues is expected to achieve precise cell type distribution, physiological cell density, perfusable vascular channels, and mature functionality. However, fabricating an engineered tissue with the microenvironmental features of organs with a physiological cell density remains a significant challenge in this field due to the disturbances caused by printing vascular channels within pre-printed tissues, the need to maintain printing precision at high cell densities, and the inability of the suspension bath to provide a suitable growth environment. Herein, we modified the rheological properties of the bioactive hydrogel by incorporating thixotropic Laponite nanoclay (LPN), and demonstrated that an optimized ratio of collagen methacrylate (ColMA) t

Abstract: sCMOS imaging devices, known for low noise, high frame rates, wide dynamic range, and high quantum efficiency, are increasingly used in spectrometers for high-speed and high-sensitivity applications. Fluctuations in their quantum efficiency can cause variations in spectral curves, leading to spurious peaks, altered peak shapes, or shifts, significantly affecting Raman spectroscopy. This study examines four quantum efficiency compensation methods, focusing on spectrum traceability, smoothness, and signal-to-noise ratio (SNR) changes. Recommendations for each method’s applicability are provided. Key findings include: (1) For smoothness, blackbody radiation compensation is best, followed by lower envelope, median, and upper envelope compensation; (2) Compensation effectiveness decre