Abstract: Vascular channels embedded within tissue-engineered hydrogels play a crucial role in replicating natural physiological environments and facilitating the delivery of nutrients and removal of metabolic byproducts. Although present techniques provide diverse strategies to create vascular channels, the flexible and scalable construction of these channels within hydrogels remains a challenge. Here, inspired by Lego assembly, an innovative modular construction strategy is introduced for developing perfusable vascular channels within hydrogels. This approach involves the customized design and fabrication of individual modules featuring diverse vascular channel architectures, which can be flexibly assembled into large-scale hydrogel constructs with hierarchical vascular channels through

Abstract: The incidence and burden of skin wounds, especially chronic and complex wounds, have a profound impact on healthcare. Effective wound healing strategies require a multidisciplinary approach, and advances in materials science and bioengineering have paved the way for the development of novel wound healing dressing. In this context, electrospun nanofibers can mimic the architecture of the natural extracellular matrix and provide new opportunities for wound healing. Inspired by the bioelectric phenomena in the human body, electrospun nanofibrous scaffolds with electroactive characteristics are gaining widespread attention and gradually emerging. To this end, this review first summarizes the basic process of wound healing, the causes of chronic wounds, and the current status of clini

Abstract: Vascularization is a critical component of tissue engineering research and is essential for enhancing the success rate of tissue construction and function. Over the past decade, researchers have explored various methods to construct in vitro vascular networks, including 3D printing, cell sphere technology, and microfluidics. Microfluidic technology has garnered significant attention due to its notable advantages in precision, controllability, flexibility, and applicability. It can be primarily classified into two modes: (i) the pre-designed mode, which involves creating vascular networks by pre-designing vascular channels and seeding endothelial cells, encompassing microfluidic chips and microfluidic spinning technologies; and (ii) the self-assembly mode, where cell spheres are f

Abstract: Effective vascularization is crucial for the success of tissue engineering and is influenced by numerous factors. The present work focuses on investigating the effect of a substance, cyanobacteria-loaded oxygen-releasing hydrogel, on vascularization and verifying the effect of photosynthetic-oxygen-releasing biomaterials containing a cyanobacteria hydrogel on angiogenesis, using the chick chorioallantoic membrane (CAM) as a model system. On the eighth day of embryonic development, cyanobacterial microspheres were placed on the CAM and maintained in a light incubator under appropriate growth and photosynthesis conditions. The effect of cyanobacterial microspheres on vascularization was evaluated from the eighth day of embryonic development. The carrier material used to prepare the

Abstract:Tissue vascularization plays a critical role in the regeneration and repair of damaged tissues. However, in certain instances of tissue injury, the pace and effectiveness of vascularization can be limited. Innovative strategies leveraging magnetic fields and magnetic nanoparticles (MNPs) are devised to enhance the efficacy of tissue vascularization. This review explores the potential of magnetic field-assisted strategies in augmenting tissue vascularization and repair. Direct application of static or dynamic magnetic fields, alone or in combination with MNPs, offers a means to modulate cellular behaviors and gene expression, thereby promoting angiogenesis and tissue regeneration. Techniques such as cell labeling, gene delivery using MNPs, and magnetic targeting have shown promise